EFFECTS OF HOT WATER–PREPARED SIDA ACUTA AQUEOUS LEAF EXTRACT SUPPLEMENTED WITH DIETS ON SERUM ENZYMES AND PROTEINS OF LAYING HENS

Abstract

One hundred and twelve (112) ISA Brown points of lay birds were randomly distributed into7 treatment groups of 4 replicates per group with 4 birds in each replicate. Seven experimental diets were formulated for the study. A corn-soy bean meal- maize offal-based diet (basal diet) was the control. The basal diet was supplemented with 2ml and 4ml of hot water-prepared Sida acuta aqueous leaf extracts (100,125 and 150mg/L). Basal diet supplemented with 2ml and 4ml/kg hot water-prepared Sida acuta aqueous leaf extract (100g/L) respectively represented T1 and T2. Basal diet supplemented with 2ml and 4ml/kg hot aqueous extract (125g/L) respectively denoted T3 and T4. Basal diet supplemented with 2ml and 4ml/kg hot aqueous extract (150g/L) respectively tagged T5 and T6. The experimental birds were not vaccinated and medicated throughout the experimental period. However, the experimental hens had access to administration of multivitamin fortnightly of 5days weekly. Phytochemicals such asphytate, flavonoids, steroids and terpenoids were in higher concentrations in hot waterprepared Sida acuta aqueous leaf extracts than those of the leaf of S. acuta. There was significant interaction (P=0.044) effect of dosage and inclusion on serum ALP with the highest in T4. It was concluded that the supplementation of 4ml/kg of 125g/L of hot water-prepared Sida acuta aqueous leaf extract with the diet elevated serum ALP. This is suggestive of detrimental effect to the health of the hens when used for prolong period