The Application of the Immunoperoxidase (IP) Antibody Technique for the Etiological Diagnosis of Infectious Bursal Disease (IBD) in Chickens

Abstract

The Indirect IP – technique was employed in demonstrating a site where IBD antigen reacted with Its untbody in IBD-affected barya of Fabricius. In the, rabbit - wot-chicken IgG, which was conjugated to borwer dish peroxidase, was used as the Immunochemical tracers of the antigen-antibody la teraction between IBD chicken underum wad wc tions of bursa of Fabricius (BF) from IBD infected chicken. The technical details were examined and compared with the immunofluorescent (IF) and body technique. The IP – technique was simpler to perform, gave permanently stained specimens, and was answer to interpret because it was less subject to background staining than the IF - technique However both the IP and the IF — techniques were of equal diagnostic sensitivity and specificity. Some attempts made to use the IP - technique for detecting IBD teld virus isolates in cell-culture solatoh yarns were unsuccessful. The IP - technique was, however, capable of demonstrating vll-culture adapted IBD viruses lo cell monotavers.